丝素
材料科学
复合数
骨整合
蛋白质吸附
涂层
模拟体液
化学工程
生物矿化
磷灰石
粘附
钛
吸附
丝绸
图层(电子)
复合材料
植入
聚合物
化学
扫描电子显微镜
冶金
有机化学
医学
外科
工程类
作者
Lu Wang,Changzhen Xu,Kejing Meng,Yijing Xia,Yufang Zhang,Jing Lian,Xing Wan,Bin Zhao
出处
期刊:ACS Biomaterials Science & Engineering
[American Chemical Society]
日期:2022-12-06
卷期号:9 (1): 165-181
被引量:1
标识
DOI:10.1021/acsbiomaterials.2c00995
摘要
Various modifications performed on titanium alloy surfaces are shown to improve osteointegration and promote the long-term success of implants. In this work, a bioactive nanostructured hydroxyapatite (HA) composite coating with a variable morphology mediated by silk fibroin (SF) and its derived peptides (Cs) was prepared. Numerous experimental techniques were used to characterize the constructed coatings in terms of morphology, roughness, hydrophilicity, protein adsorption, in vitro biomineralization, and adhesion strength. The mixed protein layer with different contents of SF and Cs exhibited different secondary structures at different temperatures, effectively mediating the electrodeposited HA layer with different characteristics and finally forming proteins/HA composite coatings with versatile morphologies. The addition of Cs significantly improved the hydrophilicity and protein adsorption capacity of the composite coatings, while the electrodeposition of the HA layer effectively enhanced the adhesion between the composite coatings and Ti surface. In the in vitro mineralization experiments, all the composite coatings exhibited excellent apatite formation ability. Moreover, the composite coatings showed excellent cell growth and proliferation activity. Osteogenic induction experiments revealed that the coating could significantly increase the expression of specific osteogenic markers, including ALP, Col-I, Runx-2, and OCN. Overall, the proposed modification of the Ti implant surface by protein/HA coatings had good potential for clinical applications in enhancing bone induction and osteogenic activity of implants.
科研通智能强力驱动
Strongly Powered by AbleSci AI