Expression of nitrite reductase gene from Lactobacillus plantarum in Escherichia coli

The expression of nitrite reductase gene from Lactobacillus plantarum in Escherichia coli was studied.Nitrite reductase gene was amplified by PCR from the genomic DNA of L.plantarum,and then inserted into expression plasmid pET-32a(+)to construct recombinant plasmid.The recombinant plasmid was transformed into E.coli BL21(DE3).The host bacteria containing recombinant plasmids was selected and grew with IPTG induction.The cell lysate of the host bacteria was extracted for SDS-PAGE and its enzymatic activity was determined.The results showed that the nitrite reductase gene was successfully expressed and the expressed protein contained nitrite reductase activity.