The Advances in the Research of Gene Knockout Mice

Gene knockout mice are desirable animal model for researching the function of gene and protein in vitro. There are some disadvantages in traditional knockout technology, such as complicated analysis of phenotype, long cycle time, numerous operations, etc, so the key problem to apply this technology better to medical research at present is how to set up knockout mice model more scientifically and more conveniently. This review introduced the latest advances in the research of gene knockout mice on several aspects, which included genetic background of embryonic stem cell, tissue-specific gene expression and innovation of gene targeting technology. [