Influence of Feiliuping Extractum and its modified formulas on function of dendritic cells

Objective To observe the interference of Feiliuping Extractum and its modified formulas on the antigen presentation of dendritic cells in cultural system in vitro and study the molecular mechanism.Methods The inducing cultural system was established to induce and differentiate mature dendritic cells in human peripheral blood mononuclear cells(PBMC),ie.PBMC were separated from peripheral blood of healthy people.The cytokines(TNFα,IL-4 and GM-CSF) were used jointly to induce and obtain the mature dendritic cells in differentiation and function.The interference of Feiliuping Extractum and its modified formulas to dendritic cells and the expressions of related surface molecules to antigen presentation were detected by using flow cytometry,and their influence on the content of IL-12 secreted by dendritic cells were studied.Results Feiliuping Extractum up-regulated the expressions of dendritic cells and related cytomembrane molecules to antigen presentation,such as MHC-Ⅱ,CD80,CD83,CD86 and CD40 and promoted the IL-12 content secreted by dendritic cells.Conclusion Feiliuping Extractum can regulate antigen presentation of dendritic cells and improve the body function of anti-tumor immunological surveillance.