The ITS sequences Comparison of several common Potentilla in Jilin province

Objective To find the differences in the sequences of several common Potentilla in Jilin Province and provide the scientific data for the identification and authentication among the species of Potentilla.Methods The genomic DNA were isolated from the laminae of several common Potetilla,and its ITS region were amplified by using ITS universal primers of angiosperm,and the purified PCR products were directly sequenced.Results There is a little differences in ITS sequences among the same species of the collected Potentilla for being a hybrid.The other species of Potentella had completely the same nucleotide sequences.There are significantly different ITS sequences among the different species of Potentilla.Conclusion ITS sequence analysis and other methods derived from it can be used in authentication among the species of Potentilla.