Extraction and Elimination of Virus of dsRNA from Pleurotus ostreatus

Taking Pleurotus ostreatus as material,the special dsRNAs of virus were extracted from Pleurotus ostreatus with modified SDS method,and elimination of virus in Pleurotus ostreatus was performed with reagent-mycelial tip-cutting and high temperature-mycelial tip-cutting methods.The results showed that the dsRNA was the same as one in foreign studies.The virus could be effectively eliminated with reagent-mycelial tip-cutting method,and the methods,including of ribavirin-mycelial tip-cutting,cycloheximide-mycelial tip-cutting,ribavirin and moroxydine hydrochloride-mycelial tip-cutting,could reduce the quantity of dsRNA of virus.The system of extraction of dsRNA was developed using tissues of Pleurotus ostreatus as material.In addition,the technique could effectively detect virus in Pleurotus ostreatus.