The lethal dose for 50%(LD50) of Vibrio anguillarum W-1 in seaperch fry and the pathogenicity of strain W-1 stored in different conditions were investigated in this paper.The results showed that the LD50 for seaperch fry with immersion infection was 2.5×107.5cell/mL while the LD50 for the seaperch fry challenged with immersion after abdominal puncture was 2.5×107cell/mL.The pathogen W-1 was an opportunistic pathogen and injury was the main infection factor.In addition,the strain W-1 reproduced many times as well as the strain W-1 stored at 15℃,-20℃ and-80℃ had a strong pathogenicity to the seaperch fry.