Effects of EGB on AGEs-induced Endoplasmic Reticulum Stress in Cardiomyocytes

Objective: To investigate the effects of EGB on advanced glycosylation end products(AGEs)-induced cardiomyocyte injury and expressions of endoplasmic reticulum stress(ERS) markers such as GRP 78 and CHOP.Methods: Cultured neonatal rat cardiomyocytes were randomly divided into control group,AGEs group and AGEs+EGB group.MTT assay was used to measure cell viability.Protein levels of GRP 78 and CHOP were surveyed by western blot assay.Results: Compared with control group,Tm group(48,72 h) resulted in a significant decrease in cell viability.Presence of EGB markedly improved cell viability,compared with AGEs group.Exposure of cardiomyocytes to AGEs resulted in significant upregulations of GRP 78 and CHOP.Co-treatment of EGB and AGEs led to attenuation of GRP 78 and CHOP.Conclusion: These findings demonstrate that EGB protects cardiomyocytes against AGEs-induced cardiomyocyte injury.It may be associated with attenuation of endoplasmic reticulum stress.