Objective To discuss the clinical application of frozen section in thyroid operation so as to raise the rate of correct diagnosis.Methods A retrospective review was made to 248 frozen sections and paraffin sections.Results The frozen section diagnosis showed out 217 cases with benign lesion,22 cases with malignant tumor and there were 9 delayed diagnosis;in the control,the paraffin section showed 220 cases with benign lesion and 28 cases with malignant tumor;There were 3 false-negative diagnosis by frozen section (1.21%) and conformed diagnosis rate of frozen section reached 95.1%.Conclusions The correct diagnosing rate of frozen section in thyroid operation may be raised by increasing the section quality and improving the method.