Extraction of Berberine from Rhizoma Coptidis

Inspired from Soxhlet extractor and with the aid of regular laboratory apparatus,a set a novel device was designed and assembled to extract berberine from rhizoma coptidis.Compared the novel device to the traditional refluxing device,Soxhlet extractor device and constant pressure funnel device,it was found that the device had the advantages of short extraction time,high extraction rate,and only in need of routine apparatus.Based on the experiment,the optimal technique to extract from 5 g rhizoma coptidis was 80 mL 95% ethanol and 2.5 h for refluxing.