On the Crystallization of Hydroxyapatite under Hydrothermal Conditions: Role of Sebacic Acid as an Additive

Hydroxyapatite (HAp) is a major inorganic component in bone minerals and is often used for bone tissue engineering. Herein, we synthesized HAp using sebacic acid as an additive at different pH values by a hydrothermal method. Sebacic acid, which has two carboxyl group ends of the carbonate chain, binds with Ca ions during the hydrothermal process to become a crystal nucleation site in (001) and at the same time could act as an inhibitor in a specific direction [i.e., (110)] for the HAp crystal growth. Sebacic acid and the hydroxyl ion (OH–) are competitively attracted to the a(b)-plane of HAp. Depending on the pH condition, the crystal growth resulted in different morphologies depending on the ratio of sebacic acid and hydroxide ions. It was confirmed through Fourier-transform infrared spectroscopy and Raman spectroscopy that dicalcium phosphate anhydrous with HPO4 was produced under acidic conditions and HAp was produced under neutral and basic conditions. The plate- and nanorod-HAp crystals' preferential growth along the c-axis, which were obtained under neutral and basic conditions, was analyzed by transmission electron microscopy. Growth control in the c-axis direction of HAp is necessary for the understanding of crystallization of bone minerals because the mineral inside the collagen fibrils in bone tissue also shows a c-axis orientation.