免疫沉淀
荧光素酶
免疫分析
抗原
抗体
分子生物学
诺如病毒
病毒学
化学
血清学
生物
转染
病毒
生物化学
免疫学
基因
作者
Christine Tin,Stanislav V. Sosnovtsev
出处
期刊:Methods in molecular biology
日期:2019-01-01
卷期号:: 137-152
被引量:2
标识
DOI:10.1007/978-1-4939-9597-4_8
摘要
The luciferase immunoprecipitation system (LIPS) assay is a liquid-phase immunoassay that quantitates antigen-specific serum antibodies by measuring luminescence emitted by the reporter enzyme Renilla luciferase (Ruc) fused to an antigen of interest. The LIPS assay can be utilized as a high-throughput and sensitive serological method for profiling serum antibodies recognizing diverse antigens. This chapter provides a detailed protocol for detecting human norovirus-specific serum antibodies with the LIPS assay.
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