Evaluation of Vitamin B1, B2 and B6 Tablets in Bangladesh by UV-Vis Spectrophotometer

Thiamine hydrochloride (vitamin B1), riboflavin (vitamin B2) and pyridoxine hydrochloride (vitamin B6) were assayed in pharmaceutical dosages form. Thiamine hydrochloride of 100 mg dose of six different companies, riboflavin of 5 mg dose of five different companies and pyridoxine hydrochloride 20 and 25 mg dosages of two different companies of Bangladesh were extracted from the tablets, cleaned up and their active ingredients were evaluated by UV-Vis spectrophotometer at 432, 445 and 292 nm, respectively. Evaluation was carried out with respect to calibration curves of their standard reference samples. Amount of thiamine hydrochloride in 100 mg tablets of six different companies were found to be in the range of 37.62±1.11 mg to 79.03±0.42 mg. For riboflavin in 5 mg tablets of five different companies, active ingredient was found in the range of 6.78±0.19 mg to 8.23±0.15 mg. In case of pyridoxine hydrochloride tablets, it was found that 20 mg tablet of one company contained 21.75±0.41 mg and 25 mg tablet of another company contained 29.72±0.59 mg of active ingredient. Recovery experiments were done by spiking excipients of the respective medicines at three different concentration levels with 5 replicate studies. Correlation coefficients (r2) were found to be 0.99 for three vitamins and recoveries were 82.26± 0.10,99.61± 0.06 and 109.91±0.12 for thiamine hydrochloride (vitamin B1), riboflavin (vitamin B2) and pyridoxine hydrochloride (vitamin B6) respectively.