Fluorescence Resonance Energy Transfer (FRET) and Single Molecule Fluorescence Detection Studies of the Mechanism of Protein Folding and Unfolding

This chapter contains sections titled: Introduction What are the Main Aspects of the Protein Folding Problem that can be Addressed by Methods Based on FRET Measurements? Theoretical Background Determination of Intramolecular Distances in Protein Molecules using FRET Measurements Experimental Challenges in the Implementation of FRET Folding Experiments Experimental Aspects of Folding Studies by Distance Determination Based on FRET Measurements Data Analysis Applications of trFRET for Characterization of Unfolded and Partially Folded Conformations of Globular Proteins under Equilibrium Conditions Unfolding Transition via Continuum of Native-like Forms The Third Folding Problem: Domain Motions and Conformational Fluctuations of Enzyme Molecules Single Molecule FRET-detected Folding Experiments Principles of Applications of Single Molecule FRET Spectroscopy in Folding Studies Folding Kinetics Concluding Remarks