底漆(化妆品)
数据库
计算机科学
计算生物学
数据挖掘
生物
化学
有机化学
作者
Wei Chang,Yue Niu,Mengna Yu,Tian Li,Jiana Li,Kun Lu
出处
期刊:Methods in molecular biology
日期:2021-11-14
卷期号:: 173-182
被引量:5
标识
DOI:10.1007/978-1-0716-1799-1_13
摘要
Real-time quantitative polymerase chain reaction (qPCR) is a powerful tool for analyzing and quantifying gene expression, and its primer design is the first and most important step. In order to improve the efficiency and effectiveness of primer design, we designed a database qPrimerDB, based on thermodynamics gene-specific for multispecies qPCR primers design.In this chapter, we explained the working principle of the database and detailed the step-by-step practical steps with examples. The valuable and time-saving qPrimerDB database is publicly accessible at http://biodb.swu.edu.cn/qprimerdb and will be routinely updated.
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