费斯特共振能量转移
荧光
体内
检出限
材料科学
光热治疗
纳米技术
核酸
荧光寿命成像显微镜
小RNA
线性范围
生物物理学
星团(航天器)
化学
生物
生物化学
色谱法
遗传学
光学
基因
物理
程序设计语言
计算机科学
作者
Zichen Liang,Changlong Hao,Chen Chen,Wei Ma,Maozhong Sun,Liguang Xu,Chuanlai Xu,Hua Kuang
标识
DOI:10.1002/adma.202107449
摘要
Here, Zirconium metal-organic frameworks @ gold (ZrMOF @ Au) cluster architectures have been fabricated and then functionalized with two fluorescent dyes (Quasar [QS] and Cyanine5.5 [Cy5.5]) through deoxyribonucleic acid hybridization, to form a fluorescence resonance energy transfer (FRET) encoded ZrMOF @ Au-QS/Cy5.5 complex. In the presence of the target intracellular microRNA (miR)-21, the fluorescence of Cy5.5 at 705 nm (F705 ) decreases and the fluorescence of QS at 665 nm (F665 ) increases when Cy5.5 is released from the surface of ZrMOF @ Au-QS/Cy5.5. The change in the fluorescence ratio (F705 /F665 ) shows an outstanding linear range of 0.006-67.9 amol/ngRNA , and the limit of detection is 4.51 zmol/ngRNA in living cells. The high ratio loading of nucleic acid on surface of ZrMOF @ Au cluster and two fluorescence encoded signal enables better sensitivity and reliability. Zeptomolar sensitivity and good linearity against target affords distinct imaging-based monitoring of the cancer marker miR-21 both in living cells and in vivo. At the same time, the architecture displays remarkable photothermal conversion efficiency (53.7%) and gives rise to outstanding therapy ability in vivo. This strategy offers new avenues for the intelligent quantification of miRNAs for simultaneous diagnoses and treatments of early-stage cancers.
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