The deviation of serum albumin level determined by bromocresol reagent in nephrotic syndrome and hemodialyzed patients

Objective To investigate the deviation of serum albumin(Alb) in patient with renal in the determination by bromocresol green(BCG)or bromocresol purple(BCP) reagent. Methods The Alb in nephrotic syndrome and hemodialyzed patient was determined by BCG,BCP assay and compared with immuno-turbidimetric method(ITM). Results In nephrotic syndrome patients,the level of serum Alb determined by BCG assay was higher than that determined by ITM and the top value of the difference was(7.8) g/L.In hemodialyzed patients,the result of BCP assay was lower about(3.1) g/L than ITM. Conclusions High concentration of α_2 globulin has obviously affected the Alb determination by BCG reagent especially when serum A/G(0.8) in nephrotic syndrome patients.In hemodialyzed patients,underestimated value appeared correlated to the 3-carboxy-4-methyl-5-propyl-2-furanpropanoic acid(CMPF) in the serum.