Analysis and Comparison of the Metabolites Produced by A Strain of Bacillus subtilis by Use of Two Different Mediums

A strain of Bacillus subtilis, with high-temperature resistance, was isolated from the production environment of Moutai Liquor. It was cultured by using wheat and sorghum as mediums respectively, and its metabilites were analyzed and compared by GC-MS. The comparison results suggested that Bacillus subtilis could produce tetramethyl pyrazine by use of both the two mediums. Among the two mediums, it was found that the strain in wheat mediun had a higher pyrazine tetramethyl content (1.86 mg /kg wheat).