Sanchinoside R1 as An Immunopotentiator of Hepatitis A Vaccine Containing Aluminium Adjuvant

Objective To explore the effect of sanchinoside R1 as an immunopotentiator of hepatitis A(HA) vaccine containing aluminium adjuvant as well as the feasibility of decreasing the aluminium content in vaccine.Methods Add the compound adjuvant consisting of aluminium hydroxide and sanchinoside R1,at various dosages,to hepatitis A virus (HAV) antigen respectively.Inject i.m.mice with the prepared HA vaccines,determine the serum HAV-IgG every 4 weeks and compare with those of mice immunized by adjuvant-free vaccine and by the vaccine containing aluminium adjuvant.Results The HAV-IgG titers of mice in various test groups reached the peak values 12 weeks after immunization then decreased gradually.The IgG titer induced by vaccine containing compound adjuvant was significantly higher than that by adjuvant-free vaccine.The addition of sanchinoside R1 at a certain dosage enhanced the effect of adjuvant and prolonged the maintain time of antibody level.Conclusion The compound adjuvant consisting of aluminium hydroxide and sanchinoside R1 enhanced the immune effect of HA vaccine and decreased the dosage of aluminium adjuvant used.