传感器
材料科学
聚合物
小分子
发光
纳米技术
生物传感器
氧气
分子
葡萄糖氧化酶
体内
化学
光电子学
有机化学
生物化学
复合材料
生物技术
物理
生物
量子力学
作者
Kai Sun,Ying Tang,Qiong Li,Shengyan Yin,Weiping Qin,Jian Yu,Daniel T. Chiu,Yubin Liu,Xuanjun Zhang,Changfeng Wu
出处
期刊:ACS Nano
[American Chemical Society]
日期:2016-06-20
卷期号:10 (7): 6769-6781
被引量:129
标识
DOI:10.1021/acsnano.6b02386
摘要
Small molecules participate extensively in various life processes. However, specific and sensitive detection of small molecules in a living system is highly challenging. Here, we describe in vivo real-time dynamic monitoring of small molecules by a luminescent polymer-dot oxygen transducer. The optical transducer combined with an oxygen-consuming enzyme can sensitively detect small-molecule substrates as the enzyme-catalyzed reaction depletes its internal oxygen reservoir in the presence of small molecules. We exemplify this detection strategy by using glucose-oxidase-functionalized polymer dots, yielding high selectivity, large dynamic range, and reversible glucose detection in cell and tissue environments. The transducer–enzyme assembly after subcutaneous implantation provides a strong luminescence signal that is transdermally detectable and continuously responsive to blood glucose fluctuations for up to 30 days. In view of a large library of oxygen-consuming enzymes, this strategy is promising for in vivo detection and quantitative determination of a variety of small molecules.
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