Studies on the inactivated oil emulsion binary vaccine against newcastle disease and fowl cholera (III)――Safety and immune efficacy test of vaccine on duck and goose

[Objective] The aim of this study is to provide theoretical basis for immunizing waterfowls(duck and goose) with the inactivated oil emulsion binary vaccine against Newcastle Disease(ND) and Fowl Cholera(FC).[Method] Bacterial liquid from solid culture media inoculated avian Pasteurella multocida(APM) type A and allantoic fluid from embryonic eggs infected with Newcastle Disease Virus(NDV) attenuated strain La Sota were mixed and inactivated by formalin to prepare 5 batches of inactivated oil emulsion binary vaccine,which were then used for the safety and immune efficacy test on duck and goose.[Result] Immunized ducks and geese didn't performed any adverse reactions in the safety test of the 5 batches of vaccine;the immune efficacy test showed that ND-HI antibody titers of ducks and geese were no less than 4 log2 three weeks after inoculation,and the protection rates against NDV and APM were 100% and 66.7%-83.3%,respectively.[Conclusion] The binary vaccine against ND and FC is safe and reliable for duck and goose,and can provide sufficient immunity protection against ND and FC.