Determination of monosaccharides in polysaccharides from the mucilage of Bullacta exarata by HPCE

Objective:The method of HPCE was applied in the analysis of the monosaccharide components of three novel polysaccharides (EBPA,EBPB1,EBPB2) isolated from the mucilage of Bullacta exarata(BE). Method:The monosaccharides were derived from a-naphthylamine and separated by HPCE in the borex buffer solution as carrier. The standard electropherogram of monosaccharides was analyzed. Polyssaccharides were hydrolyzed into monosaccharides and treated by the same method. The monosaccharides composition of polysaccharides were obtained. Result:the optimal operating conditions:75 mmol/L sodium tetraborate buffer (pH 10.4),10 kV,25 ℃,and 0.5 Psi pressure injection for 10 s. The monosaccharides compositions and molar ratios were determined. Conclusion:This method is simple,rapid,sensitive and can be used to determine the monosaccharides in polysaccharides of BE.