Cloning of Human scFv Against Soman Transition State Analogues (P6) From a Large Phage Antibody Library

Aim To clone human anti-soman transition state analogues antibodies from a large single-chain phage antibody library. Methods An organophosphorus hapten P6 [O1-methyl-O2-(1, 2, 2-trimethylpropyl)-2-hydroxy-5-nitrophenyl methylphosphonic acid] was synthesized. Its chemical conjugate with bovine serum albumin (BSA) was used as antigen (P6-BSA) to screen antibodies against soman. Panning of a large single-chain phage antibody library was conducted to select specific antibodies against soman. The antigen binding characteristics were analyzed by ELISA. Results After 4 rounds of panning, 14 clones had specific binding ability to P6. DNA fingerprinting showed that diverse specific human scFvs against P6 was obtained from the library by biopanning. Conclusion Human anti-soman transition state analogues scFvs have been cloned from large phage antibody library.