A new technology which was used to extract rapeseed oil by combining physical grinding with enzymatic degradation was put forward.The pretreatment methods of rapeseed and the enzymatic hydrolysis technology and enzymatic hydrolysis kinetics were explored.Then the function and the effect factors of neutrase in rapeseed oil extracting process were discussed The optimum enzymatic hydrolysis conditions were as follows:temperature 50℃,pH 6.0~6.5,the ratio of stuff to water 1∶4~1∶5,dosage of heutrase 200 U/g,the granularity 160~200 mesh.This new technology can provide the foundation for the optimization techniques to extract rapeseed oil using aqueous enzymatic method.