Studies on the technics of enzymatic hydrolysis of protein in scallop skirts by using endoenzymes and exopetidases

The technics of enzymatic hydrolysis of protein in scallop processing wastes-scallop skirts by using several endoenzymes, mixed endoenzymes and exopetidases are studied in this paper. The hydrolysis effect of the multi-step enzymatic hydrolysis using mixed endoenzymes (trypsinase ∶substilisin=3∶1) and Flavourzyme is the best. Through the orthogonal test, the optimum conditions for two-step enzymatic hydrolysis using Flavourzyme are determined to be as follows ∶ enzyme added 250 U/g,enzymolysis time 55 h,temperature 55℃,pH 65. The degree of hydrolysis is 682%, the concentration of free amino acids in the hydrolysate is 7840 g/L,and that of essential amino acids is 3157 g/L.