Study of the antiaging effects of bird's nest peptide based on biochemical, cellular, and animal models

Aiming for antiaging functions, an edible bird's nest peptide (EBNP) was systematically evaluated. First, it was shown that the EBNP had 6.64% sialic acid, 68.89 g/100 g amino acid content. Subsequently, it was found that EBNP had antioxidant capacity, the DPPH•, ABTS+, and •OH scavenging IC50 values of EBNP were 1.08 mg/mL, 0.46 mg/mL, and 370.43 mg/mL, respectively, and the antioxidant EC50 value of EBNP in HaCaT cells was 14.14 mg/mL. The EBNP had melanin synthesis inhibitory capacity, the melanin synthesis inhibition EC50 value of EBNP in B16 cells was 1.48 mg/mL, and the melanin synthesis inhibition EC50 value of EBNP in zebrafish was 0.11 mg/mL. In addition, the EBNP showed anti-photoaging capacity in zebrafish and cell scratch repair and proliferation capacity in MRC-5 cells. It could be concluded that the EBNP had potential to be applied in functional foods, pharmaceuticals, and skin care products as an antiaging ingredient.