模块化设计
共价键
纳米技术
机制(生物学)
计算机科学
生化工程
化学
组合化学
比例(比率)
芯(光纤)
钥匙(锁)
模型系统
工作(物理)
反应条件
共价结合
作者
Meifeng Cai,Zifu Ni,Ziqi Sun,Xue Li,Yanli Qi,Le Wang,Chao He,Cheng‐wei Li
摘要
The SpyTag/SpyCatcher system is a modular protein assembly tool. Its core mechanism is the formation of isopeptide bonds, which achieves protein assembly through covalent coupling. This system is characterized by mild reaction conditions, rapid connection and no need for additional reagents, and shows good application potential in fields such as enzyme engineering. Although the system has made progress in application, it still faces challenges such as industrial scale and clinical immunogenicity. This paper systematically reviews the principle of the SpyTag/SpyCatcher system and its development progress. It also analyzes the deficiencies of the system in industrial applications, focuses on elaborating its specific application examples in enzyme engineering, discusses existing challenges, and looks forward to future research directions. Overall, this review aims to provide references and new ideas for research in related fields.
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