Acesulfame Potassium Alleviates Food Allergy via Mast Cell Stabilization and MAPK-Mediated Immune Regulation

experiments demonstrated that AK dose-dependently inhibited mast cell degranulation and suppressed the secretion of pro-inflammatory cytokines (IL-4, IL-6, and IL-13). Western blot analysis further revealed that AK significantly suppressed the MAPK signaling pathway in activated mast cells, with reductions of 60% in p-JNK, 57% in p-ERK, and 64% in p38 phosphorylation. These findings elucidate that AK exerts dual protective effects through both mast cell stabilization and systemic immunomodulation, proposing its potential incorporation into hypoallergenic diets. The study provides novel insights into food additives' capacity to regulate hypersensitivity reactions, with implications for developing functional foods targeting immune-mediated disorders.