检出限
微分脉冲伏安法
电化学
材料科学
辣根过氧化物酶
纳米复合材料
电化学气体传感器
纳米颗粒
纳米技术
循环伏安法
线性范围
安培法
电极
一级和二级抗体
胶体金
纳米结构
碳纳米管
生物传感器
化学工程
玻璃碳
过氧化氢
伏安法
化学
复合数
铂纳米粒子
纳米材料
核化学
免疫分析
作者
So Eun Kim,Shanmugam Sureshkumar,Hak Yong Kim,Alagan Muthurasu,Jae Chol Yoon
标识
DOI:10.1021/acsabm.5c01820
摘要
A highly sensitive and selective electrochemical immunosensor was developed for the detection of the cancer biomarker alpha-fetoprotein (AFP), a key indicator of cancer. This sensor utilizes the enhanced electrochemical current response generated by a composite material consisting of gold nanoparticles (Au NPs) decorated on a metal-organic framework (MOF) containing iron and cobalt (FeCo). The Au NP-decorated FeCo-based MOF labeled with primary antibodies (Ab1) significantly enhances the electrochemical response, enabling accurate detection of AFP. Similarly, HRP-Au nanoprism (Au NPR) nanocomposites were prepared via a one-pot assembly, where horseradish peroxidase (HRP) and the secondary antibody (Ab2) were coimmobilized on Au NPRs to form a stable nanocomposite. The immunosensor was fabricated by assembling Au NPs@ FeCo-MOF and capture antibodies (Ab1) onto a glassy carbon electrode. The MOF served as a conductive matrix, AuNPs enhanced electron transfer, and Ab1 ensured specific antigen recognition. When the AFP antigen is present, labeled Ab2 binds to the Au NP-decorated FeCo-MOF via specific antigen-antibody interactions, leading to enhanced electrochemical signals for sensitive detection. The immunosensor response was measured by differential pulse voltammetry (DPV) in phosphate-buffered solution (PBS) containing hydrogen peroxide (H2O2) and 3,3',5,5'-tetramethylbenzidine (TMB). Under controlled conditions, the immunosensor exhibited a linear response to AFP over the range of 0.0001 to 100 ng mL-1, with a detection limit of 1.2 pg mL-1 (S/N = 3), indicating high sensitivity. The immunosensor's performance was validated by detecting AFP in human serum samples, demonstrating its potential for ultrasensitive detection of AFP and other biomarkers.
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