薄层荧光显微镜
光学镊子
材料科学
光学
荧光
旋转(数学)
垂直的
光学切片
显微镜
波长
光学显微镜
光轴
激发
荧光显微镜
激光器
光电子学
扫描电子显微镜
物理
几何学
数学
量子力学
镜头(地质)
作者
Jun Yin,Rongguang Liang,H.Q. Hou,Miao Yan,Liping Yu
出处
期刊:Optics Letters
[The Optical Society]
日期:2024-02-22
卷期号:49 (5): 1193-1193
摘要
We present a light sheet fluorescence microscopy (LSFM) with active optical manipulation by using linear optical tweezers (LOTs). In this method, two coaxially transmitting laser beams of different wavelengths are shaped using cylindrical lenses to form a linear optical trapping perpendicular to the optical axis and an excitation light sheet (LS) parallel to the optical axis, respectively. Multiple large-sized polystyrene fluorescent microspheres are stably captured by LOTs, and their rotation angles around specific rotation axes are precisely controlled. During a sample rotation, the stationary excitation LS scans the sample to obtain fluorescence sectioning images of the sample at different angles.
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