趋化性
ETS1型
小桶
CCL5
下调和上调
生物
趋化因子
发病机制
免疫学
抄写(语言学)
信号转导
分子生物学
转录因子
基因
基因表达
细胞生物学
受体
炎症
遗传学
转录组
T细胞
免疫系统
白细胞介素2受体
语言学
哲学
作者
Xin Yu,Lü Li,Menghao Zhang,Jinjing Liu,Hua Chen,Fengchun Zhang,Wenjie Zheng
标识
DOI:10.1016/j.clim.2022.109161
摘要
Behçet's disease (BD) is a systemic vasculitis characterized by neutrophil activation with unclear pathogenesis. This study aimed to explore the transcriptional profiles of BD neutrophils and identify specific gene signatures. We performed RNA sequencing on neutrophils from treatment-naive active BD patients and healthy controls, then analyzed differentially expressed genes (DEGs), Kyoto Encyclopedia of Genes and Genomes pathways (KEGG) and transcription regulatory network. Quantitative real-time PCR and Western Blot were used to validate chemotaxis-related DEGs expression. We detected 567 DEGs, including 520 upregulated genes and 47 downregulated genes. 9 KEGG pathways were enriched, dominated by the NF-κB pathway and chemotaxis. The transcription regulatory network suggests ETS1 regulated the enhanced chemotaxis of BD neutrophils. Validation experiments demonstrated the overexpression of ETS1, CCR6 and CCL5 in BD neutrophils compared with HC, and ETS1 was significantly increased in vascular BD compared with other BD subgroups. Our study revealed increased activation and chemotaxis of BD neutrophils characterized by the overexpression of CCL5, CCR6 and ETS1.
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