化学
纳米团簇
纳米探针
甲基丙烯酸
生物传感器
荧光
过氧化氢
催化作用
谷胱甘肽
纳米颗粒
聚电解质
光化学
组合化学
无机化学
共聚物
聚合物
纳米技术
有机化学
物理
量子力学
材料科学
生物化学
酶
作者
Yaxin Xu,Jun Yan,Yuxiang Zhu,Haoyu Chen,Cuiyan Wu,Xiaohua Zhu,Youyu Zhang,Haitao Li,Meiling Liu,Shouzhuo Yao
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2022-10-11
卷期号:94 (42): 14642-14651
被引量:23
标识
DOI:10.1021/acs.analchem.2c02832
摘要
In this work, near-infrared fluorescent silver nanoclusters (Ag NCs) were prepared based on the in situ formed poly methacrylic acid (PMAA) as the template and stabilizer, which is synthesized by methacrylic acid (MAA) and hydroxyl radical (·OH) that is generated by the cascade nanoenzyme reaction of cupric oxide nanoparticles (CuO NPs). CuO NPs possess the intrinsic glutathione-like (GPx-like) and peroxidase-like (POD-like) activities, which can catalyze glutathione (GSH) and O2 to produce hydrogen peroxide (H2O2), and then transform into ·OH. The fluorescence intensity of Ag NCs decreases with the addition of GSH, because the −SH can easily anchor on the surface, resulting in the PMAA leaving the Ag NCs, and the coeffect of GSH and PMAA results in the aggregation to form larger Ag NPs. A good linear relationship between the fluorescence quenching rate and the GSH concentration was found in the range 0.01–40 μM with the detection limit 8.0 nM. The Ag NCs can be applied in the detection of GSH in the serum, as well as bioimaging of endogenous and exogenous GSH in cells with high sensitivity. Moreover, the normal and cancer cells can be distinguished through bioimaging because of the different GSH levels. The new method for the preparation of biocompatible nanoprobe based on the nanozyme tandem catalysis and the in situ formed template can avoid the direct usage of polymers or protein templates that hinder preparation and separation, providing a reliable approach for the synthesis, biosensing, and bioimaging of nanoclusters.
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