标记法
末端脱氧核苷酸转移酶
再生(生物学)
细胞生物学
生物
细胞凋亡
平原的
生物化学
作者
Maria Rosselló,Teresa Adell
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 245-251
标识
DOI:10.1007/978-1-0716-3275-8_15
摘要
Planarians are a model animal for the study of regeneration and homeostasis. Understanding how planarians control their cellular balance is key to the knowledge of their plasticity. Both apoptotic and mitotic rates can be quantified in “whole mount” planarians. Apoptosis is usually analyzed through terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), a technique that detects cell death by identifying DNA breaks. In this chapter we detail a protocol to analyze apoptotic cells in paraffin sections of planarians, which enables a more accurate cellular visualization and quantification than in “whole mount.”
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