钙
钙显像
诱导多能干细胞
机制(生物学)
吞吐量
活体细胞成像
生物物理学
化学
细胞
生物
基因
计算机科学
生物化学
物理
胚胎干细胞
量子力学
电信
有机化学
无线
作者
Malorie Blancard,K. Ashley Fetterman,Paul W. Burridge
出处
期刊:Methods in molecular biology
日期:2022-01-01
卷期号:: 241-253
被引量:3
标识
DOI:10.1007/978-1-0716-2573-6_10
摘要
Calcium imaging is an invaluable technique to detect and characterize calcium flux in cells. The use of calcium dye provides information on the concentration and spatial distribution of calcium. Calcium imaging is a well-established technique to assess the calcium-induced calcium release mechanism in cardiomyocytes. It can also be used to characterize mutations in genes crucial for this mechanism that frequently causes arrhythmia. Here we describe a high-throughput methodology of calcium imaging that records individual calcium transients in more than 10,000 human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) in less than 30 min.
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