尿路上皮
肾炎
泌尿系统
免疫学
肾盂
趋化因子
细胞因子
狼疮性肾炎
医学
CXCL13型
炎症
病理
内科学
生物
趋化因子受体
疾病
作者
Osamu Ichii,Marina Hosotani,Abdul Masum,Taro Horino,Teppei Nakamura,Takashi Namba,Yuki Otani,Yaser Hosny Ali Elewa,Yasuhiro Kon
出处
期刊:Journal of The American Society of Nephrology
日期:2021-10-22
被引量:1
标识
DOI:10.1681/asn.2021040575
摘要
Background: Kidneys with chronic inflammation develop tertiary lymphoid structures (TLSs). Infectious pyelonephritis is characterized by renal pelvis (RP) inflammation. However, the pathological features of TLSs, including their formation and association with non-infectious nephritis, are unclear. Methods: RPs from humans and mice that were healthy or had non-infectious chronic nephritis, were analyzed for TLS development, and the mechanism of TLS formation investigated using urothelium or lymphoid structure cultures. Results: Regardless of infection, TLSs in the RP, termed urinary tract-associated lymphoid structures (UTALSs), formed in humans and mice with chronic nephritis. Moreover, urine played a unique role in UTALS formation. Specifically, we identified urinary IFN-γ as a candidate factor affecting urothelial barrier integrity because it alters occludin expression. In a nephritis mouse model, urine leaked from the lumen of the RP into the parenchyma. In addition, urine immunologically stimulated UTALS-forming cells via cytokine (IFN-γ, TNF-α) and chemokine (CXCL9, CXCL13) production. CXCL9 and CXCL13 were expressed in UTALS stromal cells and urine stimulation specifically induced CXCL13 in cultured fibroblasts. Characteristically, type XVII collagen (BP180), a candidate autoantigen of bullous pemphigoid, was ectopically localized in the urothelium covering UTALSs and associated with UTALS development by stimulating CXCL9 or IL-22 induction via the TNF-α/FOS/JUN pathway. Notably, UTALS development indices were positively correlated with chronic nephritis development. Conclusion: TLS formation in the RP is possible and altered urine-urothelium barrier-basedUTALS formation may represent a novel mechanism underlying the pathogenesis of chronic nephritis, regardless of urinary tract infection.
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