G蛋白偶联内向整流钾通道
坐骨神经
神经病理性疼痛
兴奋剂
背根神经节
感觉系统
医学
化学
神经损伤
麻醉
电生理学
膜片钳
神经科学
受体
生物
内科学
G蛋白
作者
Viola Seitz,Philip Stötzner,Dominika Łabuz,Halina Machelska
出处
期刊:Methods in molecular biology
日期:2020-09-26
卷期号:: 127-137
被引量:1
标识
DOI:10.1007/978-1-0716-0884-5_12
摘要
Patch clamp is an electrophysiological technique that allows to analyze the activity of ion channels in neurons. In this chapter, we provide a detailed description of patch clamp protocol to measure the effect of a μ-opioid receptor agonist on the activity of G protein-coupled inwardly rectifying potassium (GIRK or Kir3) channels. This is performed in peripheral sensory neurons isolated from dorsal root ganglia (DRG) of mice without or with a chronic constriction injury (CCI) of the sciatic nerve, which models neuropathic pain. We describe the induction of the CCI, isolation and culture of DRG neurons, performance of the patch clamp recordings, and identification of opioid-responding neurons.
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