纤维连接蛋白
整合素αM
整合素
细胞生物学
化学
邻近连接试验
单克隆抗体
粘附
结扎
分子生物学
生物
抗体
生物化学
流式细胞术
免疫学
受体
细胞外基质
有机化学
作者
Zaki Al‐Yafeai,A. Wayne Orr
出处
期刊:Methods in molecular biology
日期:2020-11-20
卷期号:: 17-25
被引量:1
标识
DOI:10.1007/978-1-0716-0962-0_2
摘要
Integrin activation is a crucial event for multiple biological functions. Therefore, methods to detect integrin activation are vital. Since the main cellular function of integrins is adhesion, we and others utilize this feature to measure integrin activation. Here, we describe how to detect the activity of the fibronectin-binding integrin α5β1 using a fusion of glutathione S-transferase (GST) to the 9th, 10th, and 11th type III repeats on fibronectin (GST-FNIII9-11). Moreover, we detail how to measure αvβ3 integrin activity using the ligand-mimetic WOW-1 antibody that selectively binds unoccupied αvβ3 integrins. Finally, we describe methods of testing ligation of fibronectin-binding integrins utilizing monoclonal antibodies against ligand-induced binding sites (LIBS).
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