利基
动力学(音乐)
体内
祖细胞
肌发生
神经干细胞
心肌细胞
MyoD公司
成体干细胞
生态位
作者
Brendan Evano,Sara Khalilian,Gilles Le Carrou,Geneviève Almouzni,Shahragim Tajbakhsh
出处
期刊:Cell Reports
[Elsevier]
日期:2020-03-10
卷期号:30 (10)
被引量:20
标识
DOI:10.1016/j.celrep.2020.01.097
摘要
Stem cells can be maintained through symmetric cell divisions (SCDs) and asymmetric cell divisions (ACDs). How and when these divisions occur in vivo in vertebrates is poorly understood. Here, we developed a clonogenic cell tracing method that demonstrates the asymmetric distribution of transcription factors along with old and new DNA in mouse muscle stem cells during skeletal muscle regeneration. Combining single-cell tracking and artificial niches ex vivo, we show how cells switch from ACDs to SCDs, suggesting that they are not engaged in an obligate mode of cell division. Further, we generated SNAP-tagged histone H3-reporter mice and find that, unlike fly germline stem cells, differential fate outcomes are associated with a symmetric distribution of the H3.1 and H3.3 histone variants in mouse muscle stem cells. This versatile and efficient H3-SNAP labeling system will allow an investigation of mechanisms underlying the maintenance of epigenomic identity and plasticity in a variety of tissues.
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