Antimicrobial Peptides with Protease Stability: Progress and Perspective

Future Medicinal ChemistryVol. 11, No. 16 CommentaryAntimicrobial peptides with protease stability: progress and perspectiveChangxuan Shao‡, Yongjie Zhu‡, Zhenheng Lai, Peng Tan & Anshan ShanChangxuan Shao‡Laboratory of Molecular Nutrition and Immunity, Institute of Animal Nutrition, Northeast Agricultural University, Harbin, PR China‡Authors contributed equallySearch for more papers by this author, Yongjie Zhu‡Laboratory of Molecular Nutrition and Immunity, Institute of Animal Nutrition, Northeast Agricultural University, Harbin, PR China‡Authors contributed equallySearch for more papers by this author, Zhenheng LaiLaboratory of Molecular Nutrition and Immunity, Institute of Animal Nutrition, Northeast Agricultural University, Harbin, PR ChinaSearch for more papers by this author, Peng TanLaboratory of Molecular Nutrition and Immunity, Institute of Animal Nutrition, Northeast Agricultural University, Harbin, PR ChinaSearch for more papers by this author & Anshan Shan*Author for correspondence: E-mail Address: asshan@neau.edu.cnLaboratory of Molecular Nutrition and Immunity, Institute of Animal Nutrition, Northeast Agricultural University, Harbin, PR ChinaSearch for more papers by this authorPublished Online:20 Sep 2019https://doi.org/10.4155/fmc-2019-0167AboutSectionsView ArticleView Full TextPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareShare onFacebookTwitterLinkedInReddit View articleKeywords: antimicrobial peptidesappropriate formulationchemical modificationde novo designlipopeptidePEGylationpeptidomimeticprodrugprotease stabilityunnatural amino acidReferences1. Sommer MOA, Munck C, Toft-Kehler RV, Andersson DI. Prediction of antibiotic resistance: time for a new preclinical paradigm? Nat. Rev. Microbiol. 15(11), 689–696 (2017).Crossref, Medline, CAS, Google Scholar2. Mansour SC, Pena OM, Hancock RE. Host defense peptides: front-line immunomodulators. Trends Immunol. 35(9), 443–450 (2014).Crossref, Medline, CAS, Google Scholar3. Ghosh C, Sarkar P, Issa R, Haldar J. Alternatives to conventional antibiotics in the era of antimicrobial resistance. Trends Microbiol. 27(4), 323–338 (2019).Crossref, Medline, CAS, Google Scholar4. Wang J, Dou X, Song J et al. Antimicrobial peptides: promising alternatives in the post feeding antibiotic era. Med. Res. Rev. 39(3), 831–859 (2019).Crossref, Medline, CAS, Google Scholar5. Fjell CD, Hiss JA, Hancock RE, Schneider G. Designing antimicrobial peptides: form follows function. Nat. Rev. Drug Discov. 11(1), 37–51 (2012).Crossref, CAS, Google Scholar6. Ong ZY, Wiradharma N, Yang YY. Strategies employed in the design and optimization of synthetic antimicrobial peptide amphiphiles with enhanced therapeutic potentials. Adv. Drug Deliv. Rev. 78, 28–45 (2014).Crossref, Medline, CAS, Google Scholar7. Rozek A, Powers JP, Friedrich CL, Hancock RE. Structure-based design of an indolicidin peptide analogue with increased protease stability. Biochemistry 42(48), 14130–14138 (2003).Crossref, Medline, CAS, Google Scholar8. Luong HX, Kim DH, Lee BJ, Kim YW. Antimicrobial activity and stability of stapled helices of polybia-MP1. Arch. Pharm. Res. 40(12), 1414–1419 (2017).Crossref, Medline, CAS, Google Scholar9. Cobos Caceres C, Bansal PS, Navarro S et al. An engineered cyclic peptide alleviates symptoms of inflammation in a murine model of inflammatory bowel disease. J. Biol. Chem. 292(24), 10288–10294 (2017).Crossref, Medline, Google Scholar10. Ong ZY, Cheng J, Huang Y et al. Effect of stereochemistry, chain length and sequence pattern on antimicrobial properties of short synthetic beta-sheet forming peptide amphiphiles. Biomaterials 35(4), 1315–1325 (2014).Crossref, Medline, CAS, Google Scholar11. Forde E, Schutte A, Reeves E et al. Differential in vitro and in vivo toxicities of antimicrobial peptide prodrugs for potential use in cystic fibrosis. Antimicrob. Agents Chemother. 60(5), 2813–2821 (2016).Crossref, Medline, CAS, Google Scholar12. Gomes B, Augusto MT, Felicio MR et al. Designing improved active peptides for therapeutic approaches against infectious diseases. Biotechnol. Adv. 36(2), 415–429 (2018).Crossref, Medline, CAS, Google Scholar13. She F, Oyesiku O, Zhou P, Zhuang S, Koenig DW, Cai J. The development of antimicrobial gamma-AApeptides. Future Med. Chem. 8(10), 1101–1110 (2016).Link, CAS, Google Scholar14. Forde E, Devocelle M. Pro-moieties of antimicrobial peptide prodrugs. Molecules 20(1), 1210–1227 (2015).Crossref, Medline, Google Scholar15. Walther R, Rautio J, Zelikin AN. Prodrugs in medicinal chemistry and enzyme prodrug therapies. Adv. Drug Deliv. Rev. 118, 65–77 (2017).Crossref, Medline, CAS, Google Scholar16. Hamley IW. PEG-peptide conjugates. Biomacromolecules 15(5), 1543–1559 (2014).Crossref, Medline, CAS, Google Scholar17. Bayramov DF, Neff JA. Beyond conventional antibiotics - new directions for combination products to combat biofilm. Adv. Drug Deliv. Rev. 112, 48–60 (2017).Crossref, Medline, CAS, Google Scholar18. Lam SJ, O'brien-Simpson NM, Pantarat N et al. Combating multidrug-resistant Gram-negative bacteria with structurally nanoengineered antimicrobial peptide polymers. Nat. Microbiol. 1(11), 16162 (2016).Crossref, Medline, CAS, Google Scholar19. Kim H, Jang JH, Kim SC, Cho JH. De novo generation of short antimicrobial peptides with enhanced stability and cell specificity. J. Antimicrob. Chemother. 69(1), 121–132 (2014).Crossref, Medline, CAS, Google Scholar20. Wang J, Song J, Yang Z et al. Antimicrobial peptides with high proteolytic resistance for combating Gram-negative bacteria. J. Med. Chem. 62(5), 2286–2304 (2019).Crossref, Medline, CAS, Google ScholarFiguresReferencesRelatedDetailsCited ByUnnatural amino acids: promising implications for the development of new antimicrobial peptides7 March 2022 | Critical Reviews in Microbiology, Vol. 49, No. 2Epinecidin-1, a marine antifungal peptide, inhibits Botrytis cinerea and delays gray mold in postharvest peachesFood Chemistry, Vol. 403Design, synthesis, and antimicrobial activity of novel coumarin derivatives: An in-silico and in-vitro studyJournal of Molecular Structure, Vol. 1272Synthetic amino acids-derived peptides target Cryptococcus neoformans by inducing cell membrane disruptionBioorganic Chemistry, Vol. 130Incorporation of Non-Canonical Amino Acids into Antimicrobial Peptides: Advances, Challenges, and PerspectivesApplied and Environmental Microbiology, Vol. 88, No. 23Peptide–Polymer Conjugates: A Promising Therapeutic Solution for Drug-Resistant BacteriaInternational Journal of Polymer Science, Vol. 2022Optimized proteolytic resistance motif (DabW)-based U1-2WD: A membrane-induced self-aggregating peptide to trigger bacterial agglutination and deathActa Biomaterialia, Vol. 153Strategies employed in the design of antimicrobial peptides with enhanced proteolytic stabilityBiotechnology Advances, Vol. 59Plant compounds for the potential reduction of food waste – a focus on antimicrobial peptides22 January 2021 | Critical Reviews in Food Science and Nutrition, Vol. 62, No. 15Gelatinase-Responsive Photothermal Nanotherapy Based on Au Nanostars Functionalized with Antimicrobial Peptides for Treating Staphylococcus aureus Infections28 May 2022 | ACS Applied Nano Materials, Vol. 5, No. 6Peptide-coating combating antimicrobial contaminations: a review of covalent immobilization strategies for industrial applications17 May 2022 | Journal of Materials Science, Vol. 57, No. 24In Vivo Evaluation of ECP Peptide Analogues for the Treatment of Acinetobacter baumannii Infection5 February 2022 | Biomedicines, Vol. 10, No. 2The effects of incorporation of the counterparts and mimics of l-lysine on the antimicrobial activity, hemolytic activity, cytotoxicity and tryptic stability of antimicrobial peptide polybia-MPII26 November 2021 | Amino Acids, Vol. 54, No. 1Effects of N‐terminal modifications on the stability of antimicrobial peptide SAMP‐A4 analogues against protease degradation24 May 2021 | Journal of Peptide Science, Vol. 27, No. 10Antimicrobial peptides: triumphs and challengesZi-Zhen Zeng, Shu-Hui Huang, Valérie Alezra & Yang Wan21 June 2021 | Future Medicinal Chemistry, Vol. 13, No. 16Remineralising dentine caries using an artificial antimicrobial peptide: An in vitro studyJournal of Dentistry, Vol. 111PEGylation of the Antimicrobial Peptide PG-1: A Link between Propensity for Nanostructuring and Capacity of the Antitrypsin Hydrolytic Ability1 July 2021 | Journal of Medicinal Chemistry, Vol. 64, No. 14The multifaceted nature of antimicrobial peptides: current synthetic chemistry approaches and future directions1 January 2021 | Chemical Society Reviews, Vol. 50, No. 13An optimized analog of antimicrobial peptide Jelleine-1 shows enhanced antimicrobial activity against multidrug resistant P. aeruginosa and negligible toxicity in vitro and in vivoEuropean Journal of Medicinal Chemistry, Vol. 219The multifaceted roles of antimicrobial peptides in oral diseases22 March 2021 | Molecular Oral Microbiology, Vol. 36, No. 3Insect antimicrobial peptides: potential weapons to counteract the antibiotic resistance17 February 2021 | Cellular and Molecular Life Sciences, Vol. 78, No. 9Binding loop of sunflower trypsin inhibitor 1 serves as a design motif for proteolysis-resistant antimicrobial peptidesActa Biomaterialia, Vol. 124Cross‐Strand Interaction, Central Bending, and Sequence Pattern Act as Biomodulators of Simplified β‐Hairpin Antimicrobial Amphiphiles23 December 2020 | Small, Vol. 17, No. 7Nanostructured antimicrobial peptides: The last push towards clinicsBiotechnology Advances, Vol. 44Design of a Sea Snake Antimicrobial Peptide Derivative with Therapeutic Potential against Drug-Resistant Bacterial Infection12 August 2020 | ACS Infectious Diseases, Vol. 6, No. 9Rational Avoidance of Protease Cleavage Sites and Symmetrical End-Tagging Significantly Enhances the Stability and Therapeutic Potential of Antimicrobial Peptides24 July 2020 | Journal of Medicinal Chemistry, Vol. 63, No. 17Antimicrobial peptide polymers: no escape to ESKAPE pathogens—a review1 August 2020 | World Journal of Microbiology and Biotechnology, Vol. 36, No. 9Shorter Antibacterial Peptide Having High Selectivity for E. coli Membranes and Low Potential for Inducing Resistance8 June 2020 | Microorganisms, Vol. 8, No. 6 Vol. 11, No. 16 Follow us on social media for the latest updates Metrics Downloaded 318 times History Received 27 May 2019 Accepted 13 June 2019 Published online 20 September 2019 Published in print August 2019 Information© 2019 Newlands PressKeywordsantimicrobial peptidesappropriate formulationchemical modificationde novo designlipopeptidePEGylationpeptidomimeticprodrugprotease stabilityunnatural amino acidFinancial & competing interests disclosureThis work was supported by the National Natural Science Foundation of China (31872368, 31672434); the China Agriculture Research System (CARS-35); and the Natural Science Foundation of Heilongjiang Province(TD2019C001). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.No writing assistance was utilized in the production of this manuscript.PDF download