生物
核糖核酸
RNA结合蛋白
计算生物学
核糖核蛋白
基因表达
转录组
翻译(生物学)
基因
信使核糖核酸
转录后调控
遗传学
免疫沉淀
作者
Elisha Mugo,Esteban Erben
出处
期刊:Methods in molecular biology
日期:2020-01-01
卷期号:: 285-294
被引量:4
标识
DOI:10.1007/978-1-0716-0294-2_17
摘要
Trypanosomatids rely primarily on posttranscriptional mechanisms for the control of gene expression, with regulation of RNA processing, localization, degradation, and translation by RNA-binding proteins (RBPs). To determine the mechanisms by which RBPs control gene expression in trypanosomatids, transcriptome-wide identification of mRNA targets and mapping of the RNA-binding site is required. Here we present our most current RIP-Seq (RNA immunoprecipitation followed by high-throughput sequencing) protocol that we generally apply to elucidate RNA/protein interactions in Trypanosoma brucei. The technique provides valuable information about the workings of messenger ribonucleoprotein (mRNP) networks and trypanosome gene expression mechanisms.
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