适体
检出限
肌钙蛋白I
心肌梗塞
金标准(测试)
纳米技术
生物标志物
材料科学
生物医学工程
心脏病学
内科学
化学
医学
色谱法
分子生物学
生物
生物化学
作者
Masoud Negahdary,Mostafa Behjati-Ardakani,N. Sattarahmady,Hossein Yadegari,H. Heli
标识
DOI:10.1016/j.snb.2017.05.149
摘要
Recently, convergence of nanotechnology, medicine, biology and chemistry has provided novel solutions for efficient diagnosis systems for disease markers. A detection method of troponin I (TnI), as the gold standard of acute myocardial infarction biomarker, was investigated and a novel aptasensor was presented for this biomarker. An array of gold nanodumbbells was firstly synthesized using putrescine as the shape directing agent at a surface. It was then applied as a transducer for the immobilization of a 76-mer TnI aptamer to fabricate a simple electrochemical TnI aptasensor. Using the aptasensor, TnI was detected in a linear range of 0.05–500 ng mL−1 with a limit of detection of 8.0 pg mL−1. The aptasensor showed a diagnostic sensitivity of 100% and a diagnostic specificity of 85% when challenged with blood serum samples of 89 individuals.
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