Two pathogens viz., Alternaria solani L. and Fusarium solani L. were isolated from the infected chilli fruits and their pathogenicity was confirmed. The partially purified nails component preparation was tested against these isolated pathogens. Efficacy of partially purified component isolated from human nails was tested against Alternaria and Fusarium fruit rot pathogens of chilli by poison food technique. The mycelial growth inhibition of the fungus A. solani and F. solani with nails component was 70.12% and 72.17%, at concentration 1000ppm over control. The concentration of 500 ppm was also effective (59.27% and 60.62%) but not as much effective as at concentration of 1000 ppm. The mycelial growth inhibition of the fungus A. solani was more in comparison to F. solani.