妊娠期糖尿病
微泡
胰岛素抵抗
外体
蛋白质组学
糖尿病
妊娠期
内分泌学
胰岛素
内科学
定量蛋白质组学
胎盘
怀孕
医学
生物
生物信息学
胎儿
小RNA
生物化学
基因
遗传学
作者
Nanthini Jayabalan,Andrew Lai,Soumyalekshmi Nair,Dominic Guanzon,Katherin Scholz-Romero,Carlos Palma,H. David McIntyre,Martha Lappas,Carlos Salomon
出处
期刊:Proteomics
[Wiley]
日期:2018-12-07
卷期号:: 1800164-1800164
被引量:53
标识
DOI:10.1002/pmic.201800164
摘要
Several factors including placental hormones (PH) released from the human placenta have been associated with the development of insulin resistance and gestational diabetes mellitus (GDM). However, circulating levels of PH does not correlate well with maternal insulin sensitivity across gestation, suggesting that other, previously unrecognized, mechanisms may be involved. The levels of circulating exosomes are higher in GDM compared to normal. GDM derived exosomes produce greater release of pro-inflammatory cytokines from endothelial cells compared to exosomes from normal, suggesting that their contents may differ compared to normal pregnancies. Using a quantitative, information-independent acquisition (Sequential Windowed Acquisition of All Theoretical Mass Spectra [SWATH]) approach, differentially abundant circulating exosome proteins are identified in women with normal glucose tolerance (NGT) and GDM at the time of GDM diagnosis. A total of 78 statistically significant proteins in the relative expression of exosomal proteins in GDM are compared with NGT. Bioinformatic analysis shows that the exosomal proteins in GDM target pathways are mainly associated with energy production, inflammation, and metabolism. Finally, an independent cohort of patients is used to validate some of the proteins identified by SWATH. The data obtained may be of utility in elucidating the underlying physiological mechanisms associated with insulin resistance in GDM.
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