咬边
荧光相关光谱
材料科学
荧光
半径
光学
激发
荧光光谱法
蚀刻(微加工)
激光诱导荧光
纳米技术
物理
复合材料
量子力学
计算机科学
图层(电子)
计算机安全
作者
Meiyan Wu,Wenzhao Liu,Jinyong Hu,Zhensheng Zhong,Thitima Rujiralai,Lidan Zhou,Xinlun Cai,Jie Ma
出处
期刊:Optics Express
[The Optical Society]
日期:2019-06-21
卷期号:27 (13): 19002-19002
被引量:14
摘要
The fluorescence enhancement in an over-etched gold zero-mode waveguide (ZMW) was investigated through both numerical simulation and experiments. Using Cy3 and Cy5 as the fluorescent probes, the simulation showed that the undercut not only enhances the fluorescence signals of both fluorophores, but also greatly improves the radial uniformity of the excitation fields in the ZMW. Furthermore, using a focused-ion-beam tool, we fabricated Au-ZMW arrays with different radius and undercut. The fluorescence enhancement per molecule and the effective excitation volume of the Au-ZMW were then measured as functions of its radial size and over-etching depth by using fluorescence correlation spectroscopy. It was found that the undercut can significantly enhance the fluorescence signal per molecule in the ZMW, but it also slightly increased the excitation volume. Decreasing the radial size of the ZMW can efficiently reduce the excitation volume and also further enhance the fluorescence per molecule. These results together indicate that combining the undercut and reduction of radius of the ZMW can serve as a simple and effective way to essentially improve the performance of an Au-ZMW for single molecule fluorescence detection.
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