Biocompatibility of polycaprolactone and hydroxyapatite matrices in vivo

Biocompatibility of the matrix is one of the major requirements for a functional scaffold. In this study, we carried out an assessment of biocompatibility of a complex matrix composed of polycaprolactone and hydroxyapatite under in vivo conditions by examining the dynamics of cell distribution in the original scaffold, as well as the reactions to the implant by the surrounding tissues. As result of these studies, we found that, upon subcutaneous implantation of polycaprolactone and hydroxyapatite matrix in white rats, reactive changes in the perifocal zone were completely relieved by the 21st day of the experiment. The matrix was actively populated by cells of connective tissue in the period from the 7th to 21st day of the experiment. In turn, we detected intense vascularization of the scaffold starting at the 14th day after implantation. The obtained data indicate a high degree of biocompatibility of a scaffold constructed on the basis of polycaprolactone and hydroxyapatite.