抗生素
吞吐量
注射器驱动器
微流控
抗菌剂
纳米技术
注射器
细菌生长
色谱法
生物医学工程
材料科学
细菌
计算机科学
化学
微生物学
生物
医学
电信
遗传学
精神科
无线
作者
Wenyi Zeng,Peng Chen,Shunji Li,Qiuyue Sha,Pengjie Li,Xuemei Zeng,Xiaojun Feng,Wei Du,Bi‐Feng Liu
标识
DOI:10.1016/j.bios.2022.114100
摘要
The growth of bacterial resistance to antimicrobials is a serious problem attracting much attention nowadays. To prevent the misuse and abuse of antimicrobials, it is important to carry out antibiotic susceptibility testing (AST) before clinical use. However, conventional AST methods are relatively laborious and time-consuming (18-24 h). Here, we present a hand-powered vacuum-driven microfluidic (HVM) device, in which a syringe is used as the only vacuum source for rapid generating concentration gradient of antibiotics in different chambers. The HVM device can be preassembled with various amounts of antibiotics, lyophilized, and stored for ready-to-use. Bacterial samples can be loaded into the HVM device through a simple suction step. With the assistance of Alamar Blue, the AST assay and the minimum inhibitory concentration (MIC) of different antibiotics can be investigated by comparing the growth results of bacteria in different culture chambers. In addition, a parallel HVM device was proposed, in which eight AST assays can be performed simultaneously. The results of MIC of three commonly used antibiotics against E. coli K-12 in our HVM device were consistent with those obtained by traditional method while the detection time was shortened to less than 8 h. We believe that our platform is high-throughput, cost-efficient, easy to use, and suitable for POCT applications.
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