Differentiation and Characterization of Myeloid Cells

Abstract Recent molecular studies of myeloid differentiation have utilized several in vitro models of myelopoiesis. Hematopoietic progenitors expressing the CD34 + antigen can be induced in vitro in a process that recapitulates the normal myeloid development. Two human leukemic cell lines, NB‐4 and HL‐60, have been demonstrated to undergo retinoic acid–induced myeloid development, however, both cell lines exhibit defects in the upregulation of late‐expressed neutrophil‐specific genes. In contrast, two murine factor–dependent cell models of myelopoiesis express the full range of neutrophil maturation markers: 32Dcl3 cells, which undergo G‐CSF‐induced myeloid maturation, and EML/EPRO cells, which develop into mature neutrophils in response to cytokines and retinoic acid. In this unit, the induction of myeloid maturation in each of these model systems is described. Commonly used techniques to test for myeloid characteristics of developing cells are also described. Together, these assays provide a solid foundation for in vitro investigations of myeloid development.