活体显微镜检查
双光子激发显微术
显微镜
共焦显微镜
免疫系统
CD8型
运动性
细胞生物学
体内
生物
化学
生物物理学
病理
免疫学
光学
医学
物理
荧光
生物技术
作者
Kibaek Choe,Yusaku Hontani,Tianyu Wang,Eric Hébert,Dimitre G. Ouzounov,Kristine Lai,Ankur Singh,Wendy Béguelin,Ari Melnick,Chris Xu
标识
DOI:10.1038/s41590-021-01101-1
摘要
Intravital confocal microscopy and two-photon microscopy are powerful tools to explore the dynamic behavior of immune cells in mouse lymph nodes (LNs), with penetration depth of ~100 and ~300 μm, respectively. Here, we used intravital three-photon microscopy to visualize the popliteal LN through its entire depth (600-900 μm). We determined the laser average power and pulse energy that caused measurable perturbation in lymphocyte migration. Long-wavelength three-photon imaging within permissible parameters was able to image the entire LN vasculature in vivo and measure CD8+ T cells and CD4+ T cell motility in the T cell zone over the entire depth of the LN. We observed that the motility of naive CD4+ T cells in the T cell zone during lipopolysaccharide-induced inflammation was dependent on depth. As such, intravital three-photon microscopy had the potential to examine immune cell behavior in the deeper regions of the LN in vivo.
科研通智能强力驱动
Strongly Powered by AbleSci AI