Analysis of the force between DNA molecules and substrate in AFM-based manipulation process

The force acting between molecules and substrates is crucial for the manipulation process. Atomic force microscopy (AFM) is not only a versatile tool for imaging in biological research, but also a versatile one for the manipulation of biological molecules. Because of the high mechanical resolution of AFM, it is preferred for the study of the interaction of mechanics in biological systems. This work is focused on the using of the Single-molecule force spectroscopy based on AFM (AFM-SMFS) method in the process of DNA manipulation. It explored the manipulation methods based on AFM, and then introduced one of the modification methods of the DNA molecules and the AFM cantilever. Compared with the different statements of the AFM cantilever by AFM-SMFS, analyzed the adhesion force between the molecules and the substrates.