Heating induced self-assembly denatured Bovine serum albumin film on graphene targeting biomolecules

A novel graphene biosensor based on self-assembly protein film modified graphene targeting biomolecules is presented in this literature. Bovine serum albumin (BSA) molecules in inorganic solution are self-assembled and aggregated on the graphene surface uniformly with the driving of heating for the first time. Atomic force microscope (AFM) and Raman spectra are used to investigate denatured BSA modified graphene. BSA antibody (anti-BSA) can be detected by this self-assembly denatured BSA modified graphene field effect transistor (GFET) directly with the limit of detection (LOD) of less than 10pg/ml, which indicates this new modification method could achieve highly sensitive detection of protein and may be an ideal candidate tool targeting various biomolecules in further research.